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Emma Ghazaryan

Eye Center of The Second Teaching Hospital of Jilin University
China

Title: Mesenchymal stem cells in treatment of corneal chemical injuries

Biography

Biography: Emma Ghazaryan

Abstract

Purpose of the study is to investigate the effect of mesenchymal stem cells in corneal neovascularization and wound healing and to compare the effectiveness of two possible application routs: subconjunctival injection and amniotic membrane transplantation. Materials and Methods: Animal model was made by application of sodium hydroxide. 7 days after injury animals were divided into 3 groups: Group 1 -subconjunctival injection of BMSCs, Group 2 - transplantation of amniotic membranes, previously seeded with BMSCS, and Group 3 - control group. Eyes were examined weekly with slit lamp. After 4 weeks animals were sacrificed and corneas were harvested for further examinations. Corneal flat mounts were made after ink perfusion for better vessel visualization and capturing. ELISA was performed for VEGF and MMP9. Q-PCR was used for VEGF, MMP9, TLR2 and TLR 4 genes expression. Results:Statistical analyze (One-Way Anova, SPSS) showed significant difference between groups (P=0.002, CI 95%). Difference was between group 1 and group 2 with 3. Measurement of vessels’ area also showed similar result (P=0.01, CI 95%). VEGF level and gene expression had similar pattern of difference between groups (P≤0.05, CI 95), but differences for MMP9, TLR 2 and TLR4 were not significant between groups. BMSCs were previously transduced with GFP gene by lentivirus, but none of the corneal cryosections had cells expressing GFP protein. Thus, the transplanted cells enhanced corneal would healing rather by trophic factor production and immune-regulatory effect, than by direct transdifferentiation into corneal cells. Conclusions: The aim of our study was to observe clinical effect of mesenchymal stem cell transplantation. Results showed that BMSCs enhance corneal wound healing and decrease the area of neovascularization. Further, we compared two possible application routs, and surprisingly, single subconjunctival injection appeared more effective than transplantation with amniotic membrane